Two-sample tests of proportions were used to examine differences in the diagnostic performance of the NGS assay to detect high vs. In total there were 94 type-specific infections detected in 10 samples with a median of 9. Using the LA as the gold standard, The NPV for all types was The NGS assay accurately detects multiple HPV infections in individual clinical specimens with limited sample volume and has extended coverage compared to LA. Identifying type-specific infections of human papillomavirus HPV is fundamental for understanding carcinogenic risk in epidemiologic studies, particularly in high burden areas such as sub-Saharan Africa. Some studies have shown that these broad-spectrum primer-based PCR methods may be less sensitive at detecting multiple infections [ 11 , 12 ].
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